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- VP2 gene hypervariable region VP2基因高变区
- hypervariable region of VP2 gene VP2蛋白基因高变区
- S1 gene hypervariable region I S1基因高变区I
- gene hypervariable region 基因高变区
- The VP1 and VP2 genes, under the control of SV40 promoter and CMV promoter respectively , were ligated and inserted into the gC gene region of herpesvirus of turkey (HVT) . 通过同源重组技术 ,将目的基因插入到火鸡疱疹病毒 (HVT)gC基因区 ,构建了一株含CIAVVP1、VP2基因表达单元的重组HVT(VP1VP2_rHVT)。
- According to the published canine parvovirus(CPV) VP2 gene in GenBank,a pair of primers was designed and synthesized to amplify the VP2 gene. 根据GenBank上发表的犬细小病毒(CPV)VP2蛋白基因序列,设计并合成1对引物,通过PCR扩增VP2基因全长。
- E2 protein, including hypervariable region 1 (HVR1) in the N-terminal region, contains some epitopes that can induce neutralizing antibodies. 包括高变区的E2蛋白含有中和性抗原表位,能诱导机体产生中和性抗体。
- Methods:The authors acquired VP2 gene by PCR amplifing method,and insert VP2 gere into PUC119 plasmid by cloning for sequencing. 方法 :采用PCR方法获得鸡贫血病毒的VP2基因 ,采用平端连接将其克隆到PUC119载体上进行测序 ,采用粘端连接将其亚克隆到PET载体上并进行原核表达。
- To predict antigen epitopes on B cell of VP2 of canine parvovirus 2b subtype(CPV-2b),the VP2 gene of CPV-2b was cloned and sequenced. 对犬细小病毒2b亚型衣壳蛋白VP2基因进行了克隆、测序,并用分子生物学软件DNAStar对VP2基因的推导氨基酸序列进行了表位分析。
- Objective To clarify the significance of antibodies to hypervariable region 1(HVR 1) of hepatitis C virus (HCV) in chronic HCV-infected patients. 目的 探讨慢性丙型肝炎病毒 (HCV)感染者血清中抗高变区 1 (HVR1 )抗体的多型性及其临床意义。
- Standard positive template constructing A pair of primers was designed according to the VP2 gene of PPV sequence in GenBank. 2.标准阳性模板的制备 根据GenBank中猪细小病毒VP2基因序列设计一对引物,用PCR从PPV细胞培养物中扩增出目的基因(98 bp)。
- Objective:To acquire a series of high cross_reactive hypervariable region 1(HVR1) peptides of HCV isolated in China and their cocktail. 目的 :研究丙型肝炎病毒 (hepatitisCvirus ,HCV)第一高变区 (hypervariableregion 1,HVR1)抗原的交叉反应性 ,获得适合我国HCV感染株的高交叉反应性HVR1序列及其组合。
- Aim To study the sequence diversity of the hypervariable region 1(HVR1) in the putative envelope protein E2/NS1 of genotype III/2a HCV in Chinese patients. 目的研究中国丙型肝炎患者III/2a型丙型肝炎病毒(HCV)包膜蛋白E2/NS1高变区1(HVR1)序列变异的规律及意义。
- The pCVP21 was contructed by inserting the amplified VP2 gene fragment into expression vector pCYTEXP1, containing bacteriophage promoters P R and P L in tandem preceded by the cIts 857 repressor gene. 扩增产物经双酶切后插入含噬菌体P?RP?L双强启动子的pCYTEXP1表达质粒,构建了VP2基因克隆pCVP21,经Dot?ELISA筛选出4个VP2表达阳性克隆。
- VP2 Gene of Porcine parvovirus (PPV) SD-68 strain was cloned and sequenced . The results showed that VP2 Gene of Porcine parvovirus (PPV) SD-68 strain contained 1740bp and encoded a protein of 579 amino acids . 对猪细小病毒(PPV)SD-68株VP2基因进行的克隆和序列测定表明:SD-68株VP2基因全长1740bp,编码579个氨基酸残基组成的多肽;
- Apair of primers was designed to amplify VP2 gene by PCR according to the published sequence of CPV's VP2 gene with primer5.0 software. The product of PCR named VP2 is approximate 1.8kb in length. 根据Genbank已发表的CPV-2 VP2 基因的序列;利用primer 5.;0 软件设计并合成一对引物;通过PCR 扩增出长约1
- The extremely high variability of HCV especially at the hypervariable region of its envelope protein draws back the research to develop a vaccine against it in the traditional ways. 由于HCV具有不同的基因型,基因突变率高,尤其是包膜蛋白中的高变区,使得传统的疫苗制备方法在HCV面前显得束手无策。
- Cloning and Sequence Analysis of the VP2 Gene of CPV Nanjing Isolate, CPV-GNThe present studies involve T-A cloning of major capsid- subunit-encoding VP2 gene of canine parvovirus Nanjing isolate, CPV-GN. 本研究涉及CPV-GN毒株VP2基因的克隆与序列分析。
- The invention discloses a hypervariable region 1 antigen in hepatitis C virus (HCV), and also discloses the purpose of the antigen in the preparation of hepatitis C virus detection reagent. 本发明公开了本发明提供丙型肝炎病毒(HCV)第一高变区抗原,还公开了该抗原在制备丙型肝炎病毒检测试剂中的用途。
- The NS1 gene and VP2 gene of porcine parvovirus (PPV) of HN1-strain were amplified by polymerase chain reaction (PCR). The amplified NS1 fragment of PPV was 624bp in length ; the amplified VP2 fragment of PPV was 882bp in length . 本研究利用PCR技术对猪细小病毒HN1株NS1基因与VP2基因进行扩增,分别得到624bp和882bp的基因片段。
