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- VH and VL genes of mAb E10 were ligated with human heavy chain CH1 and human CK genes. 将VH基因与人重链恒定区CH1基因连接,VL基因与人CK连接,分别构建了鼠/人嵌合重链FD基因和轻链基因。
- The VH and VL genes were inserted into a plasmid which contains a linker sequence for constructing scFv gene.The new vector named pTC scFv. 将其和先前克隆的轻链基因分别插入到一个含有连接VH和VL基因的连接序列的质粒之中,构建成单链抗体(scFv)基因的表达载体pTCscFv。
- VH and VL genes 轻重链可变区基因
- The weight of both of VH and VL, which we obtain by RT-PCR are between 250bp and 500bp. 经Rl,一PCR扩增的vH和vL基因大小位于250bP和soobp之间,vH略大于vL;
- The VH and VL fragments were assembled into scFv with a specially constructed DNA linker by PCR. 再用含Linker的引物将VH 和VL基因片段通过Linker连接起来形成scFv。
- The recombinaint plasmids of VH and VL which were transformed into E. coli BL21 (DE3) separately produced a 1.2 kD inclusion body protien upon induction with IPTG. The expression level of VH(and VL) protein was 28% (and 35%). VH和VL重组质粒分别转化到大肠肝菌BL21(DE3)中,IPTG诱导后,经SDS?PAGE电泳表明在12kD处有包含体蛋白表达,表达蛋白含量分别为28%25和35%25。
- VH is less than VOH, and VL is greater than VOL. VH小于VOH,而VL大于VOL。
- Cloning and Sequencing of VH and VL Variable Region Genes of Series Murine McAbs to Human CD Molecule 系列鼠抗CD分子单克隆抗体轻、重链可变区基因的克隆和序列分析
- Ligation of VH and VL during construction of large human ScFv antibody library 大容量人源单链抗体库构建中轻链重链可变区的连接
- There was no significant difference in M and VL. 极低频和中频变化无显著性差异。
- The sequencing show that the ScFv gene consisted of 699bp included VH with 339bp and VL with 315bp and linker with 45bp. 测序结果显示:ScFv基因由699个碱基组成,其中含339bP的vH和315bp的vL及45bP的连接肤碱基,阅读框架正确;
- Immunostaining of type IV collagen and laminin in VH and VC displayed intact basement membrane, but presented non-continuous banding type in SCC. 第四型胶原蛋白及板层素免疫组织化学染色,在疣状上皮增生及疣状癌呈现完整基底膜染色,但在鳞状细胞癌则呈现非连续的带状染色型态。
- The amplified VL and VH genes of 5E1 was 420 bp and 426 bp,respectively. 5E1VL基因全长420bp,VH基因全长426bp;
- The averaged IEMG data of the VMO and VL muscles (non-normalized or normalized) was analyzed to compare the changes of each muscle activity across exercises. 结果:不论是非标准化或标准化的肌电活动分析,皆呈现相同结果。
- I hit him fair and square on the jaw. 我不偏不倚打中他的下巴。
- It is a fact of life and you must face it. 这是严酷的现实,你必须面对它。
- She and I can't live under the same roof. 她和我不能住在一起。
- She had to scrimp and save to pay for her holiday. 她不得不为付假期的费用而苦苦攒钱。
- I'll try and rustle you up something to eat. 我设法给你弄点吃的。
- He sprang up and rushed to the door. 他跳起身,跑到门口。