Using suitable enzymes to cut the plasmid pRA-9 and pGEM-T Easy, we recovered the fragments of Actinidin gene promoter (1.3kb) and ipt gene (0.72kb). Then linked these two fragments with pBI121 plasmid and transformed it into E.

• PCR检测结果表明转化质粒pBI 121一Actinidin一iPt可以扩增出1 .；3kb明亮的目的片断，分别用Xbal与BamHI和Ba]卫Hl与Sacl双酶切转化质粒，电泳结果显示转化质粒被成功的切出了1