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- Methods Type specific primers and PCR were used to detect the HBV genotypes of 127 Uighur CHB patients in Xinjiang. 方法采用型特异性引物巢式PCR法对127例维吾尔族慢性乙型肝炎患者进行基因分型,并测序验证。
- In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes. APP 12种血清型分别具有不同的毒素基因（apxI,apxII,apxIII,apxIV）。
- After PCR anplification of total community DNA using a specific primer set,why is it usually necessary to eithercloneor run DGGE on the products before sequencing them? 相同的条带表示分子量相同但可能序列不同，需通过DGGE检测证明是一种DNA序列。
- The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence. 根据已报道的大肠杆菌不耐热肠毒素（LT）的基因序列设计引物，用PCR方法从Escherichia coli 216株基因组中扩增出LT基因的全序列。
- The ORF1 sequence of gene mrp encoding muramidase-released protein(MRP) was amplified from genomic DNA of Streptococcus suis type 2 Qinghai strain by PCR with specific primers. 根据猪链球菌2型溶菌酶释放蛋白基因(mrp)的序列,设计并合成了1对特异性引物,以青海株的基因组DNA为模板扩增了mrp基因ORF1序列。
- Objective: (1) To establish PCR reaction system that uses allele-specific primer PCR technique to detect SNP of KLOTHO gene. 目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
- Methods PCR-SSP was set up by synthesized 29 specific primers and 1 pairs of internal control primer,20 PCR reaction for DR alleles. 方法合成29个特异性引物和1对阳性对照引物,组成20个PCR反应用于DR位点,建立一步法PCR-SSP。
- The universal primer(CHS1 1S,CHS1 1R)is regarded as a specificity primer. The result showed that the sensitivity of universal primer PCR is 100 fg. 皮肤病原真菌通用引物(CHS1 1S,CHS1 1R)具有较强的特异性,敏感度为100 fg;
- Sensitivity determination of universal primer PCR: Seven samples ranging from 100 ng to 100 fg shown positive,except the 10 fg sample yielding negative band. 皮肤病原真菌通用引物PCR敏感性测定,模板DNA 100 ng~100 fg的7个系列浓度均扩增出了阳性条带,10 fg为阴性。
- Sequence specific primer ( PCR - SSP) 顺序特异性引物
- GDB could not parse a type specification output by the compiler. GDB不能分析编译器产生的某种类型的说明。
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根据GeneBank中TMV MP的序列设计基因特异引物，扩增出运动蛋白基因的整个开放阅读框（ORF）。
- Type specification complete, the quality is reliable. 种类规格齐全，质量可靠。
- Objective To investigate the possibility of applying degenerate oligonucleotide primer PCR(DOP-PCR) and comparative genomic hybridization(CGH) in analysing genomic genetics of a single cell. 目的探索单细胞退变寡核甘酸引物PCR(DOP-PCR)-比较基因组杂交(CGH)技术应用于单细胞全基因组分析及着床前胚胎遗传学研究的可能性。
- Studies have shown that nuclear matrix proteins (NMPs) are both tissue and cell type specific. 近年来研究表明，核基质蛋白具有组织和细胞特异性。
- A pair of specific primer was designed for amplification of cry2Ab gene. The amplified products of cry2Ab gene was directly inserted into pUCm-T vector and transformed into E. 根据已知的cry2Ab基因ORF序列，设计合成了一对特异性引物，PCR扩增出cry2Ab基因全长，并直接与pUCm-T载体相连。
- We also found the regulation of PTEN by E-cadherin was cell-cell adhesion dependent and cell type specific. 另外，我们还证实了，此调控是细胞-细胞粘附依赖的，且并非存在于所有的细胞类型中。
- Methods HLA-B* gene polymorphism in 31 patients with DCM and 29 normal control subjects was analyzed using polymerase chain reaction-sequence specific primer(PCR-SSP) technique. 方法采用聚合酶链反应和顺序特异性引物(PCR-SSP)基因多态性分析方法,对31例扩张型心肌病患者及29例无血缘关系健康人的HLA-B*各等位基因及亚基因进行检测分析。
- Genomic DNA of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with specific primers of delta sequence. 提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱。
- That is, their type specific implementations are relocatable/unloadable during runtime. 也就是说，这些类型的实现是在运行时重置和卸载的。
