Two pairs of specific primers were designed according to the conservative domain of PRV(gp50)and PPV(VP2). The zone of DNA amplification of single PCR appeared in 217 bp and 158 bp,respectively.

• 根据猪伪狂犬病病毒(PRV)和猪细小病毒(PPV)2种病原体的基因保守序列,分别设计并合成了与PRV的gp50基因序列和PPV的VP2基因互补的两对引物,进行单相PCR,分别扩增出预期的217bp片段及158bp片段;