To establish a simple,sensitive automatic method of determination of TIBC in serum with three reagents,Serum TIBC was determined with 5-Br-PAPAP in the presence of surfactant.

• 为建立简便、灵敏的测定血清总铁结合力(TIBC)自动化分析法,在表面活性剂存在下,用2-(5-溴-2-吡啶偶氮)-5-二乙氨基酚(5-Br-PADAP)为显色剂测定血清TIBC。