The thymus, bursa Fabricius and spleens were collected from the killed chickens, weighed, observed and conserved in formaldehyde, followed by embedding organs with wax and slicing, and ANAE staining.

• 宰杀时取胸腺、法氏囊和脾脏观察形态、称重和取材放于甲醛-蔗糖缓冲液中固定，之后取上述3种淋巴器官组织块进行常规的石蜡包埋切片和非特异性酯酶染色。