The syndromes were evaluated by means of the combined behavioral score (CBS) a couple of weeks after operations. The GAP-43mRNA expression of the motor neurons and nerve roots were monitored by SYBR Green fluorescence fix quantification RT-PCR technique.

• 术后2周按CBS评分标准检查动物神经缺失症状,用SYBRGreen荧光定量RT-PCR方法检测脊髓腹角运动神经元胞体及其神经根GAP-43mRNA的表达改变。