The study and application of ICSI in human and animal were summarized,and effects of egg,sperm,operation and others on the micro fertilization were discussed.

• 作者综述了胞质内精子注射(ICSI)技术的原理及其在人及其他动物上的研究应用,阐述卵子、精子操作技术等对ICSI结局的影响,指出目前存在的问题和应用前景。