The results revealed that the expression of intact hNT-3 gene in eukaryotic cells was regulated effectively by the Tet-Off system,and the bioactivity of the secreted proteins was detected.

• 结果显示，应用Tet-Off系统可对hNT-3全基因在真核细胞内的转录进行严格地调控，其分泌蛋白具有良好的生物活性。