The method used the suitably buffer system,neuraminidate and Triton X-100,it made the quantitive analysis bone and liver ALP isoenzyme in serum become impossible and let us satisfied to sensitivity and precision. [WT5HZ]

• :采用合适的缓冲液体系 ,使用神经氨酸苷酶和TritonX - 10 0作为辅助手段并用荧光扫描 ,使定量测定肝及骨型同工酶成为可能 ,且灵敏度和精密度令人满意。