The decalcified tissue blocks were sectioned in a mesial-distal plane, then stained with hematoxylin and eosin(HE).

 
  • 10%25甲醛心脏灌注固定,待组织固定后,取大鼠上颌骨组织,包括牙及牙槽骨,制备近远中组织切片,HE染色,应用计算机图像分析软件量化根分叉区形态学改变情况,将所有结果进行统计学分析。
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