The arylamidase (E. 0. 3.4.11.2. AAD) of mouse kidney was purified by using Triton X-100 and trypsin treatment to solubilize the enzyme, and further combined with the use of ammonium sulfate fractionation and several types of column chroma-tography.
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- 用Triton X-100和胰蛋白酶处理并结合硫酸铵分段盐析和多种柱层析;从正常小鼠肾脏纯化了芳香酰胺酶(Arylamidase、简写AAD;E.;C