The LC-MS/MS method for determining the concentration of 17- beclomethasone monopropionate(17-BMP) in Beagle dog plasma were established for BDP pharmacokinetics test using BDP as the reference. The LOQ is 0.10 ng/ml.

• 建立了LC-MS/MS方法检测Beagle犬血浆中BDP的活性代谢产物17-BMP的浓度，采用BDP为对照，解决了BDP在体内浓度低无法检测的困难，定量限为0.；1ng/ml，为水雾剂的Beagle犬体内药动学研究提供了手段。