The GATA-3 mRNA expression in pulmonary tissue was messured with reverse transcription polymerase chain reaction(RT-PCR). And the IL-5,IL-13 proteins in pulmonary tissue were detected with enzyme linked immunosorbent assay(ELISA).

• HE染色观察小鼠肺组织病理变化,逆转录聚合酶链反应(RT-PCR)法检测小鼠肺组织GATA-3 mRNA表达,酶联免疫吸附法(ELISA)检测小鼠肺组织IL-5、-13蛋白的表达。