Specific biological interactions such as antibody-antigen, streptavidin-(or avidin-)biotin, and lectin-saccharide bindings have proven to be useful for constructing layered enzyme films.

• 尽管酶多层膜的制备可以基于蛋白质和相应配体之间的特异性亲合作用，如抗原-抗体间，生物素-亲合素间的相互作用等。