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- Soft Agarose Cell Cloning Kit 软琼脂细胞克隆试剂盒
- Keywords tumor cell;soft agarose culture;differentiation;drug-resistant cell clone for chemotherapy; 关键词肿瘤细胞;软琼脂培养;分化;化疗耐药克隆;
- Research on Screening of the Antitumor Drugs and Obtaintion of Drug-resistant Clone Cell by Soft Agarose Culture 应用软琼脂培养法筛选抗癌药物及获得耐药克隆细胞的研究
- Pure red cell aplasia with thymoma: evidence of T cell clone disorder. 胸腺瘤致单纯红细胞再生障碍机制的初步探讨。
- Interspecies cloning is a new technology with the development of Somatic cell clone technology. 异种克隆技术是在体细胞克隆技术成功后发展的一项新技术。
- After the plasmid EGFP-C1 was transfected into INPC, the stable cell clone designated INPC/EGFP was isolated by G418 selection. The positive rate of EGFP was observed. 质粒EGFP-C1转染INPC后;经G418筛选;挑选细胞克隆;命名为INPC/EGFP;观察其EGFP阳性率.
- This review introduce the present situation in the technique progress, the basic theoretic research and the applications of the technology of somatic cell cloning. 本文对哺乳动物体细胞克隆技术研究现状、理论基础研究、应用等方面作一综述。
- Methods T cell clones were isolated by limiting dilution. 方法通过有限稀释法建立T细胞克隆。
- Objective To found a hybridoma cell cloning method when we established strains of hybridoma cells to F1 antigen. 目的探讨在建立了分泌抗鼠疫F1抗原的单克隆抗体杂交瘤株后,对其进行克隆化的实验方法。
- TaKaRa Agarose Gel DNA Purification Kit Ver. 本试剂盒是从琼脂糖中回收纯化DNA片段的试剂盒。
- Methods:Using cell clone tech nique,cell elect rophoresis and flow cytometer,we establish ed three sublines with different metast atic potential from human ovarian cancer li ne-SKOV3 (S1,S6 and S14). 方法:应用有限稀释法对卵巢癌细胞系SKOV3进行单细胞克隆,共分离出14个克隆亚系,根据细胞电泳率,流式细胞仪检测所得细胞周期,筛选出3个电泳率差异较大的克隆亚系(S1、S6和S14)。
- soft agarose cloning technique 软琼脂克隆法
- The present study is the first to report on the usefulness of puromycin for production of enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer. 本研究是第一次报道在体细胞克隆和胚胎移植后成功应用嘌呤霉素标记获得了转强化型绿色荧光蛋白（EGFP）基因的小猪。
- Shanghai申友biotechnology company completed the study of "primer synthesis and gene sequencing," the work of the OPG (-/+) successful heterozygous mouse embryonic stem cell cloning. 上海申友生物技术公司完成了该研究中“引物合成和基因测序”的工作，成功获得了OPG(-/+)杂合子小鼠胚胎干细胞克隆。
- RT-PCR showed that hepG2 cells were positive for B7-1 at the messenger RNA(mRNA)level. The hepG2/Hb7-1 cell clone strongly expressing B7-1(positive rate:92.2%)was picked out. 而HepG2 /hB7 1的B7 1分子阳性表达率达到 92 6%25 ,转染B7 1基因后较转染前增大了 2 8倍多。
- Soft drinks are much in demand this hot weather. 这么热的天气，软饮料需求量很大。
- He was fawning on a damsel with soft words. 他正用甜言蜜语向一位女郞献殷勤。
- Only a fraction of 15% of the T cell clones presented obvious HBcAg-specific proliferation response. 仅15%25 （6/40）的T细胞克隆呈现明显的HBcAg特异性淋巴细胞增殖反应。
- I've always had a real soft spot for him. 我一向对他挺有好感。
- Most animals are soft and fluffy when first born. 大多数动物刚出生时都很软，身上有绒毛。
