So we established three effective hammerhead ribozyme vector systems targeting +8, +36 and +71 of the VEGF mRNA, among which, the ribozyme targeting +36 site was the most efficient in all evaluations.

• 综上，我们构建的分别针对VEGF121 +8, +36,和+71位点锤头状核酶可有效地抑制VEGF的RNA水平，针对+36位设计的锤头状核酶抑制效果最好。