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- Methods The expression of MDR1 gene in 77 cases of AL patients and 10 cases of control group was detected with semi quantitative reverse transcription polymerase chain reaction (RT PCR). 方法 采用半定量逆转录聚合酶链反应（ R T? P C R），对77 例 A L病人及 10 例正常人 M D R1 基因表达进行检测。
- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- It can give the qualitative, semi quantitative, or quantitative data about the compositions of fluid inclusions quickly. 它可以快速方便地对单个包裹体进行定性、半定量乃至定量分析,且样品制备简单。
- Fluorescence quantitative PCR combined with RT-PCR technique can detect the DTC in blood of the patients with HCC. RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散的肿瘤细胞,并且以肺动脉血中阳性率最高。
- For special aims, multiple PCR, reverse PCR, quantitative PCR, in situ PCR, as well as capillary PCR, were developed. 而适合不同目的的PCR技术也得到了充分的发展,如多重PCR、反转录PCR、定量PCR、原位PCR、PCR突变、毛细管PCR技术等等。
- The expression of RAE-1 and H60 genes was measured by RT-PCR and real-time quantitative PCR. 6) BALB/C小鼠正常肝脏组织能检测到RAE一1低表达。
- Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method. 目的评价荧光定量聚合酶链式反应(FQ-PCR)检测恶性疟原虫的效果。
- Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis. 目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。
- Fluorescent real-time quantitative PCR was used to investigate some of the differentially expressed genes. 对部分筛选出来的差异表达基因使用荧光定量PCR方法检测并确认其变化；
- Conclusions Quantitative PCR is a practical method for diagnosing DMD/BMD carrier with great rapidity、accura... 结论定量PCR方法是一种准确、快速、简便的DMD携带者诊断方法。
- With semi quantitative noncompetitive RT-PCR assay, the hTERT mRNA expression in IMR90 cells was negative and the normalized hTERT mRNA expression in T24 cells was 0.06[hTERT(IOD)/GAPDH(IOD)]. 半定量非竞争RT-PCR检测IMR90和T24细胞株hTERT mRNA的表达，IMR90细胞株hTERT mRNA无表达，T24细胞株hTERT mRNA表达为0.;06[hTERT(IOD)/GAPDH(IOD)]。
- Conclusion The fluorogenic Quantitative PCR method for the detection of Plasmodium falciparum is simple and accurate. 结论 建立了检测恶性疟原虫的荧光定量PCR方法，较常规PCR技术更为简便、快速、准确，有很好的应用前景和研究价值。
- Objective:To construct recombinant plasmid pMD18-nm23-H1/p as the standards for nm23-H1 gene detection by real-time fluorescence quantitative PCR. 目的:制备用于nm23-H1基因mRNA实时荧光定量PCR检测的质粒标准品。
- Methods The HBV-DNA was detected by florescence quantitative PCR and HBV serum markers was detected by ELISA in 392 serum samples. 方法392份血清用酶联免疫吸附试验(ELISA)法测定乙肝病毒(HBV)两对半,用FQ-PCR法检测HBV-DNA含量。
- Objective: To research the application of Real-Time Quantitative PCR(RQ-PCR) in rapidly detecting pathogenic bacteria genes causing food poisoning. 摘要目的：探讨实时荧光PCR快速检测在食物中毒中病原学基因诊断的应用。
- Methods:15 cases of conjuctivitis were detected for the trachoma chlamydia by fluoroqenic quantitative PCR and super microscope diagnosis system. 方法:利用荧光定量聚合酶链反应和超高倍显微诊断系统镜检检测15例结膜炎患者的沙眼衣原体。结果:两者皆阳性5例;
- Conclusions Quantitative PCR is a practical method for diagnosing DMD/BMD carrier with great rapidity, accuracy and efficiency. 结论定量PCR方法是一种准确、快速、简便的DMD携带者诊断方法。
- Validation of a Rice Specific Gene, Sucrose Phosphate Synthase, Used as the Endogenous Reference Gene for Qualitative and Real-Time Quantitative PCR Detection of Transgenes. 一种水稻特异基因-蔗糖磷酸合成酶基因的确定,用作定性和实时定量PCR检测转基因的内标准基因。
- Methods A real time quantitative PCR(RQ-PCR) was performed to measure the bcr/abl~(P190) transcripts in the bone marrow mononuclear cells from 74 CML patients in different phases. 方法应用实时定量聚合酶链反应(RQ-PCR)检测74例CML患者骨髓单个核细胞中的bcr/ablP190转录本。
- Methods HBV DNA was detected by quantitative PCR, and HBV-M was detected by ELBA. The mean amount of HBV DNA was counted in different serological marker combinations. 方法 采用定量PCR和定性PCR方法,检测216份不同临床类型血清标本的HBV DNA,再用ELISA方法测定HBV-M,统计不同免疫指标组合的HBV DNA平均含量。
