Samples were derivatized with phthaldehyde as fluorescent derivatives. The flow rate was maintained at 1.8ml/min. The three components of gentamicin, C1a,C2,and C1 eluted as distinct peaks with retention times of 7.6,9.8 aad 12.4min respectively.

• 三羟甲基氨基甲烷(375:50:105)混合溶媒作流动相；硫酸庆大霉素与邻苯二醛(OPA)制成荧光衍生物；以1.；8ml/min的流速洗脱；用荧光检测器激发波长340nm；发射波长430nm测定；庆大霉素C_(1a)、C_2、C_1的保留时间分别为7