Relative survival(RS%),relative suspension growth(RSG%),mutation frequency at tk locus and percentages of slow growth mutant(SC%) were detected and loss of heterozygosity(LOH) of mutants were analyzed.

• 检测细胞相对存活率(RS%25)、相对悬浮增长率(RTG%25)、tk位点突变频率(M F)和缓慢生长集落的百分比(SC%25),同时对突变集落tk位点杂合性丢失(LOH)进行分析。