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- Methods: CAT gene fragment was cloned via PCR technigue,which was later exprecsed in Eschen chia coli with protein reconstruction technigue. 方法:通过PCR技术克隆CAT的基因片段并通过蛋白重组融合表达技术使其在大肠杆菌中表达。
- It is mounted in a cabinet which connects to the Still Assembly via flexible hoses. 安装在一个真空柜内并和蒸馏器模块用软管相连。
- The vector was identified via PCR and restriction enzyme analysis and was introduced into Agrobacterium tumerifacience LBA4404. 经限制性内切酶分析和PCR鉴定后利用冻融法将重组质粒pBI121-A导入根癌农杆菌LBA4404。以锦橙 (Citrus.
- Mitochondrial 16S rRNA and COI gene fragments of Portunus trituberculatus from Weifang were amplified via PCR, then the PCR products were purified and sequenced. 本文采用 PCR扩增和序列测定等技术 ,对三疣梭子蟹 (Portunustrituberculatus)线粒体DNA1 6Sr RNA和 COI基因片段进行了初步研究。
- The internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear ribosomal DNA (nrDNA) was amplified via PCR in 28 taxa of Abies Mill. 由PCR反应扩增了 2 8种冷杉属 (AbiesMill.;)
- N. coenophialum and N. lolii could be distinguished effectively via PCR amplification by using two pairs of primers FM1/R1 and F3/R3 designed on the basis of AY191830 in GenBank. 根据GenBank上AY191830的序列设计引物FM1/R1、F3/R3，两对引物分别能有效地区分N.;coenophialum和N
- Thus, geographic_distributed partners can share or contribute their design capabilities each other to complete a common product design for manufacturing and assembly via Internet/Intranet. 这样 ;在地理上分散的合作伙伴可以互相共享设计能力 ;通过因特网或企业网来完成面向制造与装配的产品设计 .
- The ribosomal internal transcribed spacer regions (ITS 1 and ITS 2) and mitochondrial gene fragments ( 16S rDNA and COI) of Crassostrea gigas were amplified via PCR, and the PCR products were ligated into T vectors, cloned and sequenced. 以相应引物经PCR扩增了太平洋牡蛎 (Crassostreagigas)的核糖体转录间区域 (ITS 1和ITS 2 )及线粒体 16SrDNA和COI基因片段。 PCR产物经T 载体连接后进行克隆和测序 ,分别得到长度为 5 4 3、791、5 30和 70 0bp的核苷酸序列。
- Jack was assigned to the assembly shop of the factory. 杰克被分配到厂里的装配间工作。
- He flew to Europe via the North Pole. 他经由北极飞到欧洲。
- He is on the way to Pakistan via the Silk Route. 他正经由丝绸之路在去巴基斯坦的旅途上。
- Keywords Hepatitis C Virus;DNA Shuffling;Reassembly via PCR;Directed Evolution; 肝炎病毒;丙型;DNA改组;重聚PCR;定向进化;
- I want to speak to you re your behaviour. [口]关于你的行为，我想跟你谈一谈。
- Robots are replacing people on assembly lines. 机器人逐渐代替了装配线上的工人。
- The head teacher told the school at assembly. 校长在全校学生大会上讲话。
- This flight is routed to Chicago via New York. 这班机是经纽约飞往芝加哥的。
- A legislative assembly is being held. 一个有关立法的会议正在召开。
- I can send him a note via the internal mail system. 我可以通过内部通信系统给他发个通知。
- People have the rights of assembly and expression. 人们有集会和发表言论的权利。
- Re your letter of 1 September ... 关於您9月1日的来信 ...
