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- Random primer PCR technique 随机引物PCR
- Objective: (1) To establish PCR reaction system that uses allele-specific primer PCR technique to detect SNP of KLOTHO gene. 目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
- Keywords Streptococcus pyogenes;Gene typing;DNA polymorphism;Random primer PCR; 关键词酿脓链球菌;基因分型;DNA多态性;随机引物PCR;
- In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes. APP 12种血清型分别具有不同的毒素基因（apxI,apxII,apxIII,apxIV）。
- Citrus genomic DNA library was bloted with the AP1 and FBP1 mixed MADS box genes probe labeled by random primer method. 以大三岛脐橙基因组DNA为材料 ,用拟南芥AP1和矮牵牛FBP1的混合质粒DNA为模板 ,采用随机引物法标记探针 ,对柑桔基因组DNA文库进行噬菌斑原位杂交。
- The particular band pattern of dark and light was observed by using random primer PRINS. 结果表明，随机引物延伸在染色体上呈现明暗相间的带纹样特征。
- The universal primer(CHS1 1S,CHS1 1R)is regarded as a specificity primer. The result showed that the sensitivity of universal primer PCR is 100 fg. 皮肤病原真菌通用引物(CHS1 1S,CHS1 1R)具有较强的特异性,敏感度为100 fg;
- Citrus genomic DNA library was bloted with the AP1 and FBP1 mixed MADS-box genes probe labeled by random primer method. 以大三岛脐橙基因组DNA为材料，用拟南芥AP1和矮牵牛FBP1的混合质粒DNA为模板，采用随机引物法标记探针，对柑桔基因组DNA文库进行噬菌斑原位杂交。
- Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. 分别应用随机引物和SOX基因兼并引物在人类染色体上进行了原位延伸标记。
- Sensitivity determination of universal primer PCR: Seven samples ranging from 100 ng to 100 fg shown positive,except the 10 fg sample yielding negative band. 皮肤病原真菌通用引物PCR敏感性测定,模板DNA 100 ng~100 fg的7个系列浓度均扩增出了阳性条带,10 fg为阴性。
- The fingerprints amplification by polymerase chain reaction(PCR) with 6 polymorphic 10-based random primers selected from 40 ones was analyzed. 从40个引物(10碱基的寡核苷酸)中筛选出6个引物,对它们的PCR扩增结果进行分析。
- Studies on the Establishment and Preliminary Application of Double PCR Technique for Detection of Malaria. 疟疾双重PCR检测方法的建立和初步应用研究。
- The comparison between PCR and random primer labeled method PCR和随机引物标记探针的方法比较
- Method DNA polymorphic microsatellites of macaca rhesus were analyzed using PCR technique in 20 macaca rhesuses. 方法利用聚合酶链反应(PCR)扩增技术对20只恒河猴群个体间进行了DNA多态性的分析。
- Methods Using HE stains,Niles stains,transmission electron microscope,immunohistochemistry and RT PCR technique etc. 方法采用HE染色、尼氏染色、透射电镜、免疫组化及RT-PCR技术。
- Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA. 目的：消减文库构建过程中，用PCR技术快速筛选重组阳性克隆。
- Objective To investigate the possibility of applying degenerate oligonucleotide primer PCR(DOP-PCR) and comparative genomic hybridization(CGH) in analysing genomic genetics of a single cell. 目的探索单细胞退变寡核甘酸引物PCR(DOP-PCR)-比较基因组杂交(CGH)技术应用于单细胞全基因组分析及着床前胚胎遗传学研究的可能性。
- Methods: Optimal reaction system and single random primer of RAPD were used for genotyping of the 108 strains of EA isolated from the clinical samples and the genotype profile was drawn. 方法:以优化的反应体系和单一随机引物对临床分离的108株EA进行RAPD法基因分型并绘制基因分型图谱。
- Objective To study the application of PCR technique in genetic detection of Duchenne/Becker muscular dystrophy (DMD/BMD). 目的应用PCR技术检测假肥大型肌营养不良(DMD/BMD)基因缺失和杂合子。
- In this experiment, differential expressing genes in leg muscle of chicken mentioned above were studied via DDRT-PCR technique, which firstly adopted 72 primer pairs that piloted by 3 T7 (dT11) N as anchor primers and 24 M13-ARPs as random primers. 本实验采用3个26bp的T7(dT11)N为锚定引物,24个26bp的M13-ARPs为随机引物,组成72个引物组合对上述四个品种雏鸡腿肌组织进行DDRT-PCR反应。
