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- In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH. 4 7份ELISA检测HCV抗体阳性血清标本 ;RT nested PCR检出阳性标本 33例 (70 .;2 1%25 );RT PCR DDH检出阳性标本 39例 (82
- Methods By RT PCR DDH,RT nested PCR,RNA DDH,we detect the nucleic acid of HCV and deduce the specificity,sensitivity and stability of RT PCR DDH in viral nucleic acid detection. 方法 通过RT PCR DDH ,RT nested PCR和HCVRNA DDH 3种方法检测HCV核酸 ,推断RT PCR DDH技术检测病毒核酸的特异性、敏感性和稳定性。
- Keywords hepatitis C virus（HCV）;HCV genome;3 DNA signal amplification;dendrimer DNA hybridization（DDH）;reverse transcription nested PCR（RT nested PCR）; 丙型肝炎病毒;基因组;3DNA信号放大;树状DNA杂交（DDH）;逆转录巢式PCR;
- 5.Methods By RT PCR DDH,RT nested PCR,RNA DDH,we detect the nucleic acid of HCV and deduce the specificity,sensitivity and stability of RT PCR DDH in viral nucleic acid detection. 核酸与各类物质的相互作用与探测核酸的结构与功能密切相关是揭示核酸的生物功能和一些药物的作用机制的重要途径。
- Methods The mRNA from normal lungs was used as template. By using the techniques of RT PCR, nest PCR and cloning, the pUC 19/SP A 6 A clone was established and sequenced. 方法以正常人肺组织mRNA为模板 ,采用逆转录反应 (RT) PCR、巢式 PCR及基因克隆技术 ,获得pUC19/SP A 6A克隆 ,并测序证实。
- Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR. 利用套式PCR技术检测母血中胎儿SRY基因的研究
- Nested PCR requires two sets of primers which are used to amplify a specific DNA fragment using two separate runs of PCR. 巢式PCR引所需要两套用于放大特定的DNA片段,用两条挤提聚合酶链。
- Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established. 获取正常男性单个淋巴细胞和无DMD家族史的单个胚胎细胞,行DMD基因外显子17、19、44、45、48的五重巢式PCR,分析扩增成功率、假阳性率和假阴性率。
- In addition, there were 2 negative samples in PPD test, while they were positive in nested PCR assay and also in FQ-PCR assay. 在对2份PPD检测阴性而巢式PCR检测阳性的临床样本的检测结果也为阳性。
- Two pairs of primers were dsigned from cholera toxin subunit A(ctx A)gene and a nested PCR that can detect O1 classical (CVC),El Tor (EVC)and O 139 especially was established. 针对霍乱弧菌肠毒素A亚单位（ctx?A）基因设计两对引物，建立套式PCR，可特异扩增霍乱弧菌O1群古典型、埃尔托型和O139群；
- Methods Test 91 samples of confirmed and/or possible cases and 25 samples of healthy volunteers simultaneously by combining multiplex nested PCR with real-time PCR. 方法应用多重巢式PCR联合荧光实时定量方法，对广州地区91份确诊和疑似病例标本、25份健康志愿者标本同时进行检测。
- Method HCMV DNA and anti HCMV IgM were simultaneously tested by nested PCR using two primer sequences from HCMV IEA genome and capturing ELISA respectively in sera from 688 patients with sexually transmitted diseases and 138 blood donors. 方法 在 HCMV IEA基因内自行设计两对引物 ,建立套式 PCR检测 688例常见性病患者及 1 38名健康献血员血液HCMV- DNA,同时应用捕获 ELISA检测 HCMV- Ig M。
- Methods The ORF2 gene of TTV was amplified from the serum of a blood donor in Lanzhou region by nest PCR and inserted into vector pGEM-T then,after identification by DNA sequencing,subcloned to expression vector pET22b(+). 方法通过巢式PCR,从一份兰州地区无偿献血员血清中扩增TTV病毒的ORF2基因,并克隆到pGEM-T载体中,进行核苷酸序列分析。
- X gene (HBx), enhancer II (Enh2) and basic core promoter (Bcp) of HBV in sera from HCC patients were amplified by nest PCR , and sequenced and then compared with the sequences in Genbank. 对HCC患者血清的HBV的HBx、Enh2与Bcp基因的巢式PCR产物直接测序，与Genbank中已发表序列进行对比分析。
- Objective To establish a technique of single cell 5-plex nest PCR for Ducheme muscular dystrophy(DMD) detection and evaluate the possibility of using for preimplantation genetic diagnosis (PGD). 摘要目的：探索建立单细胞多重巢式PCR检测杜氏肌营养不良症(DMD)基因外显子17、19、44、45、48的技术，及应用于植入前遗传学诊断(PGD)的前景。
- Objective: To establish a technique of single cell 5-plex nest PCR for Ducheme muscular dystrophy (DMD) detection and evaluate the possibility of using for preimplantation genetic diagnosis (PGD). 目的:探索建立单细胞多重巢式PCR检测杜氏肌营养不良症(DMD)基因外显子17、19、44、45、48的技术,及应用于植入前遗传学诊断(PGD)的前景。
- Studies on the Rapid Detection of Foot and Mouth Disease Virus Using One Step RT PCR and Nested PCR 运用RT-PCR一步法和Nested-CR法快速检测FMDV的研究
- The swallows nested under the eaves. 燕子在屋檐下筑巢。
- In order to find out TTV infection in different types of hepatitis, the TTVDNA in serums of 97 cases was detected by nests PCR. 目的:探讨本地区不同型别肝炎患者血清中TTV感染状况。方法:采用巢式PCR对97例不同型别肝炎患者血清中TTVDNA进行检测。
- Keywords embryoid body;nested PCR;clock genes; 胚胎小体;巢式聚合酶链反应;时钟基因;单细胞;
