RT-PCR was used to estimate the relative mRNA amounts of estrogen receptor(ER),MTA3 and snail in the cells. Western blotting and gelatin zymography assay were used to estimate the relative protein amounts of MMP-2,MMP-9 and E-cadherin in the cells.

• 采用RT-PCR技术检测雌激素受体(ER)以及与侵袭有关的MTA3、Snail基因mRNA的表达,采用蛋白印迹法和明胶酶谱法检测E-钙黏附蛋白(E-cadherin)和基质金属蛋白酶2(MMP-2)和9(MMP-9)蛋白的表达。