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- An "agg" codon between 1039-1041bp in PLF gene was replaced with a "taa" stop codon through primers design and PCR. 猪乳铁蛋白基因1039-1041bp处为agg密码子，在设计引物时突变为taa终止密码子。
- The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used. 摘要引物设计是RAMP和REMAP标记分析的关键，二者都使用锚定的微卫星序列引物。
- ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis. 通过引物设计、PCR、基因克隆、测序,获得了10种鸢尾属植物和1个外类群种的ITS序列。
- We can't use these designed primers as primers designed on the mutated-type site of Pinb-D1b. 这几种突变体的筛选上,不能像 Pinb-D1b 突变体一样根据突变位点设计引物使用。
- The RT-PCR was done with the primers designed according to the sequence of PRM1 of tufted deer. 结果表明PRM1 基因仅在睾丸组织中表达，而其他组织不表达。
- PCR primers design for gene chip 基因芯片的PCR引物设计
- In China these viruses share high homology with Takahaski’s strains which might be good standards for primer designation. 因而，设计pcr引物时宜选择Takahashi等分离株为标准。
- Furthermore, with the two primers designed according to this assembled cDNA, the full-length cDNA of rice ribosomal protein was cloned by RT-PCR and named as OsRPS7. 采用计算机拼接和RT-PCR方法克隆了水稻胞质核糖体蛋白基因的全长cDNA序列，命名为OsRPS7。
- The 1.5kb long anticipated DNA fragment coding for P-450 protein was amplified by PCR, using a pair of low G/C%content primers designed according to CYP6A1 cDNA gene. 再以总cDNA为模板，以P-450 CYP6A1 cDNA序列为参考设计一对低G/C%25含量引物，进行PCR扩增，获得1.;5kb左右的预期目的片段。
- In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes. APP 12种血清型分别具有不同的毒素基因（apxI,apxII,apxIII,apxIV）。
- The prion protein(PRNP) genes of 25 Amur tigers were amplified by PCR using primers designed from mammalian PRNP. These PCR products contain 402 bp encoding 134 amino acids,and have a homology of 99.67%. 根据已报道的哺乳动物朊病毒基因序列设计引物;采用PCR方法扩增了25只东北虎的朊病毒基因;克隆、测序及序列分析表明;所得到的东北虎朊病毒基因片段为402bp;编码134个氨基酸的前体蛋白;核苷酸序列同源性为99.;67%25。
- Children learn knowledge from primers. 孩子们从启蒙书上学习知识。
- Baculovirus anti-apoptotic genes Op-iap and p35 were cloned from pIEl-4opiap and pIEl-4p35 using PCR with three pairs of primers designed according to the encoding region sequences. 依目的基因编码区序列设计了三对引物,从昆虫表达载体pIE1-40piap和plE1-4p35中扩增了细胞凋亡抑制基因iap和p35。
- Methods: The acrB gene of Salmonella typhi 275,OM2 and 182 were identified by PCR with the primers designed from Genebank(No. AL627267) and the DNA fragments were sequenced to confirm the existence of acrAB gene. 方法 :参考伤寒沙门菌基因库序列 (No .;AL6 2 72 6 7)设计引物;对伤寒沙门菌 2 75、OM 2、182PCR扩增acrB基因;以证实acrAB外排系统的存在;并对 3株伤寒沙门菌扩增所得的acrB测序并分析其序列。
- Even an oversight in the design might issue in heavy losses. 设计中那怕是一点点疏忽也可能造成重大的损失。
- Methods:The whole acrAB gene of Salmonella typhi 275 were amplified by PCR with the primers designed from Genebank,and the sequence of products and the amino acid sequence were detected. 方法 :以伤寒沙门菌基因序列为参考设计引物 ,以PCR法对伤寒沙门菌 2 75acrAB全序列进行调取并测序。
- Total RNA was abstracted from zebrafish livers with Trizol reagent, and its ratio of A260/A280 was between 1.8 and 2.0. Using RNA as a template, we conduct the RT-PCR with vitellogenin special primers designed by ourselves. 用Trizol一步法提取总RNA;A260/A280均在1.;8-2
- The method that finds Resistance Gene Analogs (RGA) using the degenerate primers designed based on the conserved motifs of plant disease resistance genes (R) was named as RGA method. 植物抗病基因(Resistance Gene,R)产物存在保守结构域,如NBS、LRR、STK、TIR、TM和LZ等。 根据这些保守序列设计简并引物寻找R基因同源序列(Resistance Gene Analogs,RGA)的方法称为RGA法。
- NS genes of muscovy duck reovirus S14 and C4 were amplified by RT_PCR with the primers designed by the reported avian and muscovy duck reoviruses. The PCR products were cloned to the pMD18_T vector and identified by enzyme cutting analysis. 参考GenBank禽呼肠孤病毒 (AvianReovirus,ARV)和番鸭呼肠孤病毒 (MuscovyDuckReovirus,MDRV)非结构基因 (NS)序列设计合成一对引物 ,对番鸭呼肠孤病毒S14和C4株NS基因进行RT_PCR扩增 ,克隆到pMD18_T载体中 ,并对克隆产物进行酶切鉴定和测序 ;
- There is an inherent weakness in the design. 这设计本身存在弱点。
