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- genetically engineered Pichia methanolica 重组甲醇毕赤酵母
- Effect of Cultivation Condition on Heterologous Expression of Lignin Peroxidase in Pichia methanolica 培养条件对甲醇毕赤酵母异源表达木质素过氧化物酶的影响
- Keywords heterogenetic expression;lignin peroxidase;Pichia methanolica;expression optimization; 异源表达;木质素过氧化物酶;甲醇毕赤酵母;表达优化;
- Keywords ABA binding protein,degenerate primer,RACE,Pichia methanolica expression system,affinity chromatography; 脱落酸结合蛋白;简并引物;RACE;酵母表达系统;亲和层析;
- Pichia methanolica 甲醇毕赤酵母
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- The similarity of CICC 1859 and Pichia anomala CBS 5759T was 100%. CICC1859与异常毕赤酵母(Pichiaanomala)CBS5759T相似性为100%25,鉴定为异常毕赤酵母。
- Pichia stipitis Xylitol dehydrogenase (XDH) Gene XYL2 was amplified by PCR. 通过PCR方法克隆得到树干毕赤氏酵母木糖醇脱氢酶(XDH)基因XYL2。
- Chen GQ, Wang HT, Li ZG and Wu Q. Expression and purification of human Tyrosinase in Pichia pasteuris. 人酪氨酸酶在毕赤巴斯德酵母中的表达和纯化。
- In Basal Salts Medium, the yeast grows up to A600=76 inoculated 10% seed pichia pastoris with A600=8-12 in 24h. 在无机盐培养基中,按10%25接种A600=8-12的种菌后,24小时酵母菌增殖至A600=76;
- AIM: To express human anti-keratin Fab in Pichia pastoris secretively and optimize the expression condition. 目的:用巴氏毕赤酵母表达抗角蛋白抗体Fab段并优化表达条件。
- The HRPC3 gene was expressed successfully by using Pichia pastoris as host strain and the activity of it is 56 IU/L. 应用毕赤酵母作为受体菌,成功表达了HRPC3基因,其活性为56 IU/L。
- Intracellular expression of cGHcGH eDNA was cloned into the yeast Pichia paslOri. 1 cGH在毕赤酵母细胞内的表达
- By analyses of the SDS-PAGE and Western blotting,it was showed that cGH protein was expressed in Pichia pastoris GS115. 对重组酵母进行诱导表达,SDS-PAGE和Western印迹分析,结果表明cGH在毕赤巴斯德酵母GS115菌株中获得了高效表达。
- SDS-PAGE and Western blot confirmed that GDNF was expressed in Pichia culture medium and silkworm larvae hemulymph. SDS-PAGE和蛋白质印迹杂交结果证实了酵母培养上清液及家蚕幼虫血淋巴中含有GDNF蛋白。
- Pichia pastoris is one of the most used expression systems for the high level expression of heterologous proteins. 巴斯德毕赤酵母是目前应用最广泛的外源蛋白表达系统。
- Pichia pastoris expression system is a recently developed system to efficiently express foreign protein. 摘要毕赤酵母表达系统是近年来发展起来的一种高效表达外源基因的表达系统。
- Objective:To express ancrod,a snake venom thrombin-like enzyme,in methylotrophic yeast Pichia pastoris. 目的:利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
- Cai R.Study on the Production of S-Adenosylmethionine by Recombinant Pichia pastoris. [4]蔡瑞.;从重组毕赤酵母中制备腺苷甲硫氨酸的研究
- The two-step fermentation conditions of opioid peptide expressed in Pichia pastoris were studied. 研究了由毕赤酵母胞内两步发酵法表达活性小分子阿片肽的条件。
