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- The paper studies and analyses the electrophoresis results of polymorphic fragments of genome DNA in adults of periodic Brugia malayi(B.malayi) from four endemic areas by RAPD. 本文采用RAPD技术对我国湖北古城、贵州独山、福建建阳和安徽泾县四个地区的周期型马来丝虫成虫基因组DNA的多态性进行扩增分析。
- AMPLIFICATION, CLONING AND SEQUENCE ANALYSIS OF CYSTEINE PROTEASE GENE FROM PERIODIC Brugia Malayi 周期型马来丝虫半胱氨酸蛋白酶基因的扩增、克隆及序列分析
- Keywords Periodic Brugia malayi;Paramyosin;Eukaryotic expressing vector;COS cells; 周期型马来丝虫;副肌球蛋白;真核表达质粒;COS细胞;
- Keywords Periodic Brugia malayi;Cysteine protease;Gene cloning;Sequence analysis; 关键词周期型马来丝虫;半胱氨酸蛋白酶;基因克隆;序列分析;
- Periodic Brugia malayi 周期型马来丝虫
- Conclusions:This method is practicable and reliable for gene detection of Brugia malayi larvae in mosquito. 结论:该技术具有良好的实用性,可用于蚊体内马来丝虫幼虫的基因检测。
- Abstract: Objective:To establish a new method for gene detection of Brugia malayi larvae in mosquito and use it in filariasis surveillance. 摘 要: 目的:探索蚊体内马来丝虫幼虫基因检测的新方法,并用于丝虫病监测。
- Abstract:Culex pipiens pallens is the mosquito vector of a number of human pathogens such as Wuchereria bancrofti, Brugia malayi, and epidemic encephalitis B virus. 淡色库蚊尤其是雌蚊不但骚扰和叮刺人畜,而且能传播丝虫病和流行性乙型脑炎,对人类的健康危害很大。
- Chemical and immunoactive characterizations of Brugia malayi adult and microfilaria, Setaria digitata adult crude antigens (BmA Ag, BmMf Ag and SdAAg) were analysed by SDS PAGE, histochemical staining and ELIB technique respectively. 应用SDS聚丙烯酰胺凝胶电泳(SDS-PAGE)和酶联免疫印迹(ELIB)技术,分析了马来丝虫成虫抗原(BmA-Ag)、微丝蚴抗原(BmMf-Ag)及牛指状腹腔丝虫成虫抗原(SdA-Ag)化学及免疫学特性。
- Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with 32P and used as probe.The B.malayi larvae in mosquito was detected with dot blot. 方法:利用基因工程技术合成马来丝虫寡核苷酸片段,经32P标记后作为探针,以斑点杂交法检测蚊体内马来丝虫幼虫。
- Lymphadenitis due to Brugia malayi 马来丝虫致淋巴结炎
- Lymphangitis due to Brugia malayi 马来丝虫致淋巴管炎
- Keywords Gene detection;Probe;Brugia malayi larvae; 基因检测;探针;马来幼丝虫;
- Studies on Gene Detection of Brugia malayi Larvae in Mosquito 蚊体内马来丝虫幼虫的基因检测
- Keywords Brugia malayi;Meriones unguiculatus;animal model; 关键词马来丝虫;长爪沙鼠;动物模型;
- Keywords Cytotoxicity;Brugia malayi;microfilariae;amino acid; 细胞毒;丝虫;马来;微丝蚴;氨基酸;
- MORPHOLOGICAL STUDIES OF THE ADULT OF BRUGIA MALAYI (BRUG, 1927) BUCKLEY, 1960 马来丝虫Brugia malayi(Brug,1927)Buckley,1960成虫形态的观察
- That period was a bright page in the history of Chinese literature. 那个时期是中国文学史上光辉的一页。
- Let us hope that the period of this injustice will be over soon. 让我们希望这个不公正的时期很快结束吧。
- Development of microfilariae of Brugia malayi to the sausage form in vitro 马来丝虫微丝蚴体外培养发育到腊肠期蚴