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- A~(1,2)BO~(1,2) genotyping by PCR sequence specific primer PCR-SSP法检测A~(1,2)BO~(1,2)血型基因型
- Sequence specific primer ( PCR - SSP) 顺序特异性引物
- Donors and recipients HLA class I typing was performed using complement dependent cytotoxicity (CDC) test with special monoclonal tray (SMT) and HLA class II gene typing by micro sequence specific primers polymerase chain reaction (Micro PCR SSP). 采用补体依赖性细胞毒试验 (CDC)和微量序列特异性引物聚合酶链反应 (Micro PCR SSP)技术进行HLA I类和II类分型。
- Polymerase chain reaction- sequence specific primer 聚合酶链反应-序列特异引物法
- Sequence specific primer based polymerase chain reaction, PCR-SSP 运用序列特异性引物聚合酶链反应
- Polymerase chain reaction with sequence specific primer, PCR-SSP 应用聚合酶链反应-序列特异性引物方法
- polymerase chain reacation sequence specific primer (PCR-SSP) 聚合酶链反应-序列特异性引物（PCR-SSP）
- The sequence specific primers of the gene mutation of the mannose combining agglutinator gene exon I at 52 site condon was detected with polymerase chain reaction. 进行甘露糖结合凝集素外显子I52位密码子基因突变的序列特异性引物聚合酶链式反应检测。
- The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence. 根据已报道的大肠杆菌不耐热肠毒素（LT）的基因序列设计引物，用PCR方法从Escherichia coli 216株基因组中扩增出LT基因的全序列。
- Keywords sequence specific primer;polymerase chain reaction;genotyping polymorphism; 序列特异性引物;多聚酶链反应;基因多态性;
- In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes. APP 12种血清型分别具有不同的毒素基因（apxI,apxII,apxIII,apxIV）。
- sequence specific primer 序列特异的引物
- After PCR anplification of total community DNA using a specific primer set,why is it usually necessary to eithercloneor run DGGE on the products before sequencing them? 相同的条带表示分子量相同但可能序列不同，需通过DGGE检测证明是一种DNA序列。
- Methods HLA-A and B alleles polymorphism in 65 patients with leukemia and 48 normal subjects were determined by PCR with sequence specific oligucleotide probe (PCR-SSO). 方法采用序列特异性寡核苷酸探针杂交技术(PCR-SSO)对65例白血病患者和48例正常对照组健康者进行HLA-A、B基因分型。
- Genomic DNA of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with specific primers of delta sequence. 提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱。
- The ORF1 sequence of gene mrp encoding muramidase-released protein(MRP) was amplified from genomic DNA of Streptococcus suis type 2 Qinghai strain by PCR with specific primers. 根据猪链球菌2型溶菌酶释放蛋白基因(mrp)的序列,设计并合成了1对特异性引物,以青海株的基因组DNA为模板扩增了mrp基因ORF1序列。
- Methods DNA samples from 183 random healthy individuals in Han population, 41 in Dai population and 66 in Uygur population were genotyped by using the polymerase chain reaction sequence specific oligonucleotide probing (PCR SSOP) method. 方法 采用聚合酶链反应 -序列特异的寡核苷酸探针杂交 (polymerase chain reaction andsequence- specific oligonucleotide probing,PCR- SSOP)方法 ,分析 183名汉族、4 1名傣族和 6 6名维吾尔族正常人群的 MICA胞外区等位基因多态性。
- Methods HLA-A, B alleles polymorphism in 197 cases with ALL and 5841 normal umbilical cord blood controls were determined by PCR with sequence specific olignucleotide probe(PCR-SSO). 方法采用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSO)方法,对197例北方汉族儿童ALL患者和5841例健康脐带血样本HLA-A,B等位基因多态性进行研究。
- Methods PCR-SSP was set up by synthesized 29 specific primers and 1 pairs of internal control primer,20 PCR reaction for DR alleles. 方法合成29个特异性引物和1对阳性对照引物,组成20个PCR反应用于DR位点,建立一步法PCR-SSP。
- Evaluating the polygene polymorphism by sequence specific primers polymerase chain reaction in large samples 用序列特异性引物多聚酶链反应的方法进行大样本、多基因的多态性鉴定
