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- Mouse GM-CSF and B7.1 cDNA were spliced through a linker sequence byover-lap extention PCR and the mGM-B7.1 fusion gene was also ligated into theretrovirus plasmid pLXSN and its sequence was analyzed. 通过 Lipofectamine 2000 介导的转染法将逆转录病毒载体 pLXSN 及构建于逆转录病毒载体上的小鼠 GM-CSF、B7.;1 基因 cDNA 和mGM-B7
- Keywords human UGRP1 gene;promoter;overlap extention PCR;site-directed mutagenesis; 子宫球蛋白相关蛋白1基因（UGRP1）;启动子;重叠延伸PCR;定点诱变;
- Overlap - extention PCR 重叠延伸PCR
- overlap extention PCR 重叠延伸PCR
- The new race track is nearly six miles in extent. 这条新跑道将近六英里长。
- There is no question of overlap between the two courses. 这两门课程之间不存在重叠的问题。
- There is a small overlap between the two circles. 在这两个圆周有一小部分重叠。
- Local assembly will help sale to some extent. 在当地组装将在某种程度上促进产品销售。
- I was amazed at the extent of his knowledge. 我对他知识的渊博感到惊奇。
- I agree with you to a certain extent, but ... 我在一定程度上同意你的意见，但。。。
- Of boundless or immeasurable size or extent. 浩瀚的在尺度或范围上无边界的或无法测量的
- I was very tired. To a lesser extent so was he. 我累极了。他也很累,但比我稍好些。
- He's in debt to the extent of 200. 他负债达200英镑。
- Short in time, duration, length, or extent. 短的指时间、期间、长度或广度短的
- Dominance by location; extent of view. 控制定位控制; 观察的范围
- The two storage rings overlap closely. 两个储存环节紧密重合。
- Price have changed to a considerable extent since last year. 自去年以来，价格有了相当大变动。
- The roofing slates were laid to overlap each other. 石板屋瓦是相叠着放置的。
- Of medium or average quantity or extent. 中等的中等的或一般的数量或范围的
- Sometimes physics and chemistry overlap. 有时很难区分出究竟是物理学还是化学。
