One day old boving blood was employed in the test by Polystan pump(group A) and Cobe pump (group B). TMB method was used to measure the level of plasma free hemoglobin.

• 用Polystan 泵（A 组）和Cobe 泵（B组）转流库存1 d ACD 血400 m l，用联苯胺显示法测定游离血红蛋白，用Koller氏法计算溶血指数。