Objective Specifity and sensitivity of a PCR assay of detecting Brucella DNA with th ree pair primers of cod-ing Br. abortus one and the same prote in gene sequence of different amplic ation fragment (that is 330bp?230bp and 223bp)were studied.

• 目的探讨编码牛种布氏菌同一蛋白基因序列不同扩增片断(分别为330bp、230bp和223bp)的3对引物检测布氏菌DNAPCR试验的特异性和敏感性。