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- The mRNA expression of ubiquitin in rat EDL muscle was determined by northern blot analysis. 应用northernblot方法测定伸指长肌组织泛素mRNA的表达变化 ,分析骨骼肌蛋白降解与泛素mRNA表达的关系。
- Kumark Saivithriy S. MRNA levels of Ca2+-independent form of protein kinase C in poslinchemic gerbil brain by northern blot analysis [J],J Biol Chem, 1997,252(61):7603. 卢布峰;鲁友明;黄诒森;蛋白激酶C对大鼠脑缺血海马突触体谷氨酸摄取的调控作用[J].;生物化学杂志;1994;10(3):371
- We obtain 15 differentially expressed genes to human glioma thruogh four times hybridizations and scanning. Northern blot analysis confirmed 436F11 clone was over expression in human brian tissue and low expression in human glioma tissues. 通过四次基因芯片筛选 ,获得 15条与胶质瘤相关的新基因 ,经northernblot证实 436F11基因在人正常脑组织中高表达 ,而在人脑胶质瘤中低表达。
- The expression of the novel gene in 12 specimens of pancreatic cancer and normal tumor-surrounding pancreas tissues and 4 pancreatic cancer cell lines(PaTu8988,SW1990,BxPC-3,AsPC-1)were detected by RT-PCR and Northern blot analysis. 应用RT-PCR和Northernblot检测新基因在12例胰腺癌和癌旁正常胰腺组织以及4种胰腺癌细胞株(PaTu8988、SW1990、Bx-PC-3、AsPC-1)中的表达情况。
- Improvements on the Methods of Plant RNA Isolation and Northern Blot Analysis, and Effects of Plastid Signal on Nuclear Gene Expression as Well as Leaf Morphogenesis 植物RNA分离与Northern杂交方法的改进及质体信号对核基因表达和叶片形态建成的影响
- Methods Northern blot analysis was used to study the expression of c myc and c fos m RNA in human embryo lung(HEL)cells exposed to cigarette smoking solution(CSS) and chrysotile(CH)separately or simultaneously; 方法 用Northernblot测定了烟溶液 (CSS)与温石棉 (CH)单独及联合作用后人胚肺 (HEL)细胞c fos和c myc癌基因mRNA水平的表达。
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 对这13个克隆进行测序和Blast同源序列分析表明,其中一个Northern杂交阳性克隆与autotaxin基因同源,另一个与钙周期蛋白基因同源。
- Northern and Western blot analysis in pMR-FG1 and pBI-FG1transformants showed that pMR-FG1 can improve the gene expression both intranscription level and in translation level. 对部分 pMR-FG1 和 pBI-FG1 转化植株的 Northern blot 和 Westernblot 分析证明,pMR-FG1 转化植株中融合蛋白的表达在转录水平和翻译水平明显高于 pBI-FG1 转化植株。
- This CTB gene product was also verified by Western blot analysis. Western blotting结果确认了该条带为CTB基因的产物。
- The results of western blot analysis were in accordance with those of 2-DE. 对部分差异蛋白采用Western-blot方法在蛋白水平的验证结果与2-DE结果基本相符。
- Northern blot showed that PBP-Harm and GOBP2-Harm are specifically expressed in the antenna of H.armigera. Northern杂交结果表明:PBP-Harm和GOBP2-Harm都在棉铃虫触角中特异性表达。
- PAI-1 protein secretion by mesangial cells was measured by Wes tern blot and PAI-1 mRNA by both RT-PCR and Northern blot. RT-PCR和Northern杂交检测PAI-1mRNA表达;
- With RT-PCR and Northern blot methods, the expression patterns of mNTKL-BPl were observed in the normal tissues of mouse. 用RT-PCR和Northern blot方法观察到mNTKL-BP1基因在小鼠的正常组织中均有表达。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA检测转基因马铃薯病毒抗性数据显示,温室条件下,3个转基因马铃薯株系(2M、3M、11M)具有病毒抗性,3个株系(1M、5M、10M)感病;
- Northern blot -- An electroblotting method in which RNA is transferred to a filter and detected by hybridization to32 P-labelled RNA or DNA. 北方转渍法--一种电子转印方法,将RNA转移至滤纸上,加上要试验的放射性标志RNA或DNA,以进行杂合,以自动显影技术观察
- RT-PCR, Western blot analysis and FACS were used to detect the expression of P2X7 receptor in these clones. 荧光分光光度计检测P2X7受体介导的胞内钙离子浓度变化。
- Using morphology observation, the RT-PCR and Western blot analysis to investigate and analysis the sub-type of CnA. 通过病理形态学检查、RT-PCR实验测定及Western blot分析确认CnA亚型。
- Animals were killed at day 3,7,14and 28 after BLM administration. The total RNA was extracted from the lung tissue. The expressions of SPsmRNA were analyzed with Northern blot. 方法气管内灌注博莱霉素A5,复制肺纤维化模型,分别于3、7、14和28天处死动物,提取肺组织总RNA,进行Northern杂交。
- The samples were examined delicately to investigated the expression of LeY oligosaccharide by Western blot analysis. 应用Western免疫印迹法,观察Ley寡糖抗原的表达。
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 为了探究内皮素?1(ET?1)对肾功能的影响和作用方式,采用斑点杂交和原位杂交方法对大鼠缺血60分钟再灌注肾组织ET?1及其受体亚型(ETA、ETB)的基因表达进行了研究。