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- In the experiment of RT-PCR detection for NDV strain B95, the result showed that it had the specific to strain B95 and the sensitivity is 0.0043ng/ul. 结果证明,用该引物可以扩增出一条310bp的特异核酸带,敏感性为0.;0043ng/ul。
- Results Restrictionendonuclease analysis showed that is a specific cloning plasmid of contained thenucleocapsid protein gene of NDV strain V4. 结果酶切分析表明重组质粒中含有新城疫病毒V4株核衣壳蛋白基因。
- Compared NP. P and M gene of strain Changchun with the published NDV strains (i. g. La Sola, B1). 与发表的NDV La Sota、B1等毒株相关基因相比较.
- Three pairs of specific oligonucleotide primers for amplifying the NP, P and M gene of NDV were designed according to the published relevant sequences of NDV, and the NP, P and M gene of a virulent NDV strain (Changchun) were amplified by RT-PCR. 计算机辅助设计了3对特异性引物.;应用RT-PCR一次性扩增出NDV长春株NP、P、M基因的氨基酸编码区(ORF)。
- Three Infectious Bronchitis Virus strains H 120 ,H 52 ,MA 5 were analyzed by the IBV NDV interference test 1)Whether to be active or inactive,IBV can interfere the HA activity in embryonating chicken eggs(EcE) reinoculated with NDV strain. IBV毒株H120、H52、MA5对NDV-LaSota的干扰实验证明IBV特异性干扰NDV增殖。 1)采取不同顺序的同胚接种法:IBV接种之后再接种NDV;
- Comparing the displayed-peptide of CLONE 20 and HN sequences of NDV strains such as F48E8 and La Sota, it was inferred that WF and QAR may play a major role in the antigenicity of HN. 同时用在线蛋白分析工具预测F48E8、La Sota毒株HN的抗原表位,展示肽有部分序列位于预测表位内。
- This strain of wheat can grow during a cold spring. 这种小麦可以在寒冷的春天生长。
- Cloning and Sequence Analysis of NP,P,M Gene of Avirulent NDV Strain Changchun 新城疫病毒长春株NP、P、M蛋白基因的克隆与序列分析
- He finally break down under the four-hour's strain. 在四小时的压力下他终于支持不了。
- Paying all the bills is a strain on my resources. 支付所有这些费用是我经济上的一大负担。
- Her speech continued in the same dismal strain. 她以悲伤的语调接著往下说。
- Could you stand the strain? Oh, that's nothing. 这样累你受得了吗?这没什么。
- He broke under the strain of heavy work. 他在繁重工作的压力之下累坏了。
- There's a strain of madness in the family. 那一家人都有点疯疯颠颠的。
- He was breaking up under the strain. 他劳累过度,身体逐渐衰弱。
- The official was warned not to strain his power. 那个官员受到警告,不可滥用职权。
- He pitched his story in a sentimental strain. 他把故事写得带有伤感情调。
- Strain began to tell on his health. 过度劳累开始影响他的健康。
- We'll finish the job at full strain. 我们将竭尽全力来完成此项工作。
- We should strain every nerve to do the work. 我们应该竭尽全力来做这项工作。
