Methods:LPS induced iNOS mRNA expression, production of NO and effects of activated macrophages on anti tumor were investigated separately by RT PCR, nitrate reductase method and 3HTdR incorporation.

 
  • 方法 :应用RT PCR、硝酸还原酶法、[3H]TdR掺入法分别观察LPS诱导的巨噬细胞iNOSmRNA表达、NO产生以及巨噬细胞抗肿瘤细胞毒作用。
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