Methods: Cholesterol(50.00 mg/L) was used on human umbilical endothelial cell line,ECV304,48 h later,cell genomic DNA methylation level was detected by HPLC.

• 方法:体外培养人脐静脉血管内皮细胞ECV304；以50.；00 mg/L的胆固醇作用于ECV304 48 h；提取细胞基因组DNA；用高效液相色谱法(HPLC)检测基因组DNA甲基化水平。