Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.

• 方法:大量提取纯化重组真核表达质粒pVAX1-GRA8,瞬时转染培养的vero E-6细胞,RT-PCR和免疫印迹法检测GRA8在细胞中的表达。