Methods The cells were cultured in DMEM/F12 medium. The proliferation and differentiation of cultured cells were studied by colony forming efficiency (CFE), immunohistochemistry staining and Western blot examination.

• 方法采用DMEM和F12培养基进行兔角膜上皮细胞培养，用克隆形成率（colony?formingeficiency，CFE）、免疫组化染色和蛋白质免疫印迹等方法检测细胞的增殖力和分化表达状况。