Methods Isogenic C57BL/6 female mice were immunized with male spleen cells as a serum donor in the search for specific antibodies against H-Y antigen.

• 方法以纯系C57BL/6雄鼠脾细胞做为抗原,通过腹腔注射免疫雌鼠的方法制备H-Y抗血清。