Methods Genomic DNA was isolated with kit, and the mitochondrial NADH dehydrogenase 1(ND1) and cytochrome c oxidase I (COI) gene fragments were amplified by polymerase chain reaction(PCR) and sequenced.

• 方法 试剂盒抽提基因组总DNA后 ,以特异性引物对线粒体还原型烟酰胺腺嘌呤二核苷酸 (NADH)脱氢酶 1(ND1)和细胞色素c氧化酶I(COI)进行PCR扩增 ,将PCR产物分别测序 ,并以生物信息学方法加以比较 ,构建系统进化树。