Methods 416 specimens were collected from December 2004 to April 2005 and inoculated in BacT/Alert 3D system(0.5ml) and on two Lowenstein-Jensen(L-J) slants(0.1ml each),respectively. Smears were stained by auramine O.

• 方法收集从2004年12月至2005年4月的临床标本共416份,每份标本接种BacT/Alert 3D系统的同时接种两支改良罗氏培养基和涂片作荧光染色。