Method Confocal laser scanning microscopy (CLSM) was combined with fluorescencedouble-labelingtechnique to observe the position a nd expression of PTEN.

• 方法采用激光共聚焦扫描显微镜光学切片和荧光探针的双重标记技术对三株细胞中PTEN的表达和分布情况进行检测。