METHODS: The C5aR expressions on ERM tissue and cultured RPE cells were detected by ABC immunohistochemical staining with mouse anti human C5aR mcAbs.

• 另取新鲜人眼球 ,常规分离、培养人RPE细胞。 以小鼠抗人C5aR单克隆抗体作为检测抗体 ,对PVR患者的ERM组织及培养的人RPE细胞做细胞免疫化学ABC染色 ,检测C5aR的表达。