Linoleate isomerase(LI ) gene was amplified by PCR from chromosome of Lactobacillus reuteri PYR8,and was inserted into pET30a to construct the prokaryotic expression vector pET30a-LI.

• 以Lactobacillus reuteri PYR8菌株基因组为模板,利用PCR方法扩增出亚油酸异构酶(linoleate isomerase,LI)基因,亚克隆到pET30a中构建原核表达载体pET30a-LI,并转化入大肠杆菌(Escherichia coli)BL21(DE3)中。