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- Methods:The plasmid pcDNA3 containing the wild type p16 gene and neo gene was used in the transfection experiment. 方法：采用pcDNA3质粒进行转染，该质粒含有野生型P16基因片段和新霉素抗性基因。
- The subclone bladder carcinoma cell lines,stably expressing bcl-2 and neo gene respectively,were successfully selected, named as BIU87/bcl-2 and BIU87/neo. 建立分别稳定表达bcl-2基因、新霉素抗性基因(neo)的膀胱癌亚克隆细胞株BIU87/bcl-2、BIU87/neo。
- Additionally, an insertion vector pM-AAT was constructed by cloning the mAAT gene into the modified pLoxP-1.4K-4.3K vector, downstream of the neo gene and upstream of the right homology arms. 将mAA基因序列连接到酶切位点被修饰的pLOxp·1.;4K43K A体的Mo基因后、同源右臂前，得到一个插入型打靶载体pM·AAT。
- The integration of neo gene had been identified through the SYBR荧光定量PCR初步确定neo基因的模板拷贝数很高。
- In order not to be late, she cut across the fields. 为了不迟到，她抄近路穿过田野。
- 290 bp Neo gene band was discovered by nest primer RT-PCR in all the samples. 套式RT -PCR检测Neo基因 ,第 2轮后全部出现 2 90bp条带 ;
- It irks us to wait for people who are late. 等候迟到的人使我们厌烦。
- The Presentation of neo Gene in Chimeric Mice Obtained from ES Cells 通过胚胎干细胞途径获得的嵌合体小鼠中neo基因在各组织中的分布
- The late boy made a beeline for his classroom. 迟到的男孩抄捷径跑去上课。
- I have a fear that we will be late. 我怕我们要迟到了。
- The dean returned in the late afternoon. 系主任是在傍晚时分回来的。
- Do you ever take the subway alone late at night? 你有没有在深夜单独乘过地铁？
- We shall find out the truth early or late. 我们迟早会查明事实真相。
- Oversleeping is a lame excuse for being late. 睡过头不是迟到的充足理由。
- My parents always frown on late nights out. 我父母向来不赞成深夜外出。
- This music's easy on the ear late at night. 这音乐夜晚听起来很好听。
- You'd better look sharp or you'll be late. 你得赶快，不然就晚了。
- It was not our fault that we were late. 这次迟到不是我们的错。
- He keeps late hours and gets very little sleep. 他睡得很晚，而且只睡一点儿时间。
- It's very rare for him to be so late. 他很少迟到。
