It was found that there were a low contamination ratio with about 4. 5% in all the axillary buds were sterilized in 0.1% HgCl2 solution for 10min, in this way the phenomenon of browning could be well avoided.

 
  • 外植体材料用0.;1%25的HgCl_2灭菌10min后接种,可使污染率降低至4
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