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- Method:Using Western blot method to measure HSP of hypothalamus in febrile and hypothermal rats. 方法：取高、低体温大鼠下丘脑组织，应用蛋白印迹法测定热休克蛋白含量。
- M ethod:Using Western blot method to measure HSP of hypothalamus in febr ile and hypothermal rats. 方法:取高、低体温大鼠下丘脑组织,应用蛋白印迹法测定热休克蛋白含量。
- The expression state of p53, Bcl-2 and Bax of rat hepatocytes BRL-3A as exposed to microcystin LR was studied with western blot method. 用蛋白印迹法研究了微囊藻毒素LR(MCLR)暴露时大鼠BRL-3A细胞p53、Bcl-2和Bax的表达情况.
- The cDNA probe hybridized with BVDV RNA, HCV RNA and yeast tRNA by the dot blot method The BVDV RNA and HCV RNA were positive and yeast tRNA was negative. 琼脂糖凝胶电泳结果说明它与BVDV RNA相似。 该cDNA的探针用斑点杂交方法与BVDV RNA,HCV RNA和酵母tRNA杂交,BVDV RNA和HCVRNA显阳性反应,酵母tRNA显阴性反应,结果说明合成的cDNA为BVDV RNA的cDNA。
- In the present study, the effects ofglutamic acid and acetylcholine (ACh) on the induction of hsp70 mRNA in PC12 cellswere studied by Northem blot method. 本文以诱导型热休克蛋白（hsp）70的cDNA为探针，运用Northernblot的方法，在嗜铬细胞瘤细胞（PC12）上，分析了谷氨酸和乙酰胆碱对hsp70mRNA的诱导作用。
- The relationship between certain protein expressions(MLH1,ERCC1,PARP,RAD51,XRCC4) and CQ increased radiosensitivity in radioresistant MDA-MB 231 cells was examined by Western blot method. 用W estern b lot印迹杂交法检验与DNA修复有关的5种基因(MLH1,ERCC1,PARP,RAD51,XRCC4)蛋白表达与CQ增加辐射抗拒细胞辐射敏感性的关系;
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。
- With RT-PCR and Northern blot methods, the expression patterns of mNTKL-BPl were observed in the normal tissues of mouse. 用RT-PCR和Northern blot方法观察到mNTKL-BP1基因在小鼠的正常组织中均有表达。
- The expression of CLC-3 mRNA and CLC-3 protein were tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blot methods. 应用逆转录聚合酶链反应（RT-PCR）、免疫印迹（Western blot）方法检测CLC-3 mRNA和蛋白的表达。
- METHODS: We used immunohistochemical ABC and Western blot method to detect the expression of p38 MAPK in lung tissues and airway epithelial cells in acute lung injury rat model and observed the influence of N acetylcysteine (NAC) on the expression. 方法 :采用免疫组织化学ABC法和Western blot技术 ;检测磷酸化的 p38MAPK在大鼠急性肺损伤模型气道和肺组织的表达 ;并同时观察N 乙酰半胱氨酸 (NAC)对其表达的影响 .
- The method seems good but it needs to be tried out. 这个方法似乎不错，但需要试验一下。
- Methods By using Northern blot and Western blot methods,the expression of gene and protein of VEGF-A,-C in all 6 tested cell lines (ASPC-1,CAPAN-1,MIA-PaCa-2,PANC-1,COLO-357 and T3M4) was detected respectively. 方法 用Northern杂交和Western杂交法分别分析人胰腺癌细胞株(ASPC 1、CAPAN 1、MIA PaCa 2、PANC 1、COLO 3 5 7和T3M 4)中VEGF A、C基因和蛋白的表达;
- Methods Using RT-PCR and Western Blot methods,the changes of AMPA receptor GluR2 subunit mRNA and protein expressions were observed in cultured hippocampal neurons of rats induced by glutamate. 方法用RT-PCR和Western Blot方法观察谷氨酸诱导培养大鼠海马神经元AMPA受体Glu R2亚单位mRNA和蛋白的表达变化。
- Phage Immuno Blot Screening Kit 噬菌体免疫杂交筛选试剂盒
- In this study the authors detected both nm23 H 1 and nm23 H 2 mRNA levels in 52 tissues samples of patients with carcinoma of buccal mucosa (CBM) by Northern blotting method. 通过Northern斑点杂交技术研究52例颊癌及相关组织中肿瘤转移抑制基因nm23－H1和nm23－H2mRNA的表达。
- How can we blot out the memory of past misery? 我们怎能忘记过去的苦难呢？
- How can we blot out the memory of past misery. 我们怎能忘记过去的苦难呢？
- That new factory is a blot on the landscape. 那新建的工厂破坏了此地的景色。
- I can't claim infallibility for this method. 我不敢说这方法绝对有效。
- Analyses of the changes of Calbindin-D28k(CB) expression and Glial Fibrillaiy Acidic Protein(GFAP) expression in rats hippocampus using method immunohistochemistry and western blotting method. 应用免疫组织细胞化学方法和western印迹技术分析大鼠海马结构的神经元内维生素D依赖性钙结合蛋白D28k(Calbindin-D28k,CB)和神经胶质细胞内胶质纤维酸性蛋白(Glial Fibrillary Acidic Protein, GFAP)表达的变化。
