Guanosine overproducing mutant was derived from Bacillus subtilis T1001 by using ultraviolet and diethyl sulfate (DES). The mutant was selected directionally and step wisely on the agar plates containing methionine sulfoxide(MSO) and 8 azaguanine(8 AG).

• 以枯草芽孢杆菌T1 0 0 1为出发菌株 ,经紫外线、硫酸二乙酯逐级诱变处理 ,选育出腺嘌呤缺陷型 (Ade- )菌株 ,然后经蛋氨酸亚砜 (MSO)、8 氮鸟嘌呤 ( 8 AG)结构类似物平板定向筛选 ,获得鸟苷高产菌株TA2 0 8。