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- The green fluorescence protein (GFP) gene recombinant virus labeling is a new method for the neuroanatomical studies. 绿色荧光蛋白(GFP)基因重组病毒标记技术是神经解剖研究的新方法。
- The renaturation of gene recombinant protein is the most important technique in the field of biotechnology which's many methods have been established. 基因重组蛋白质的复性是当前生物工程领域的重要研究课题。
- CHEN Xiuyun,CHEN Chunpo,WU Vujuan,et al.Study on the estrogenic activities of DDT pesticides based on the gene recombinant yeast cell[J].J Toxicol,2006,20(6):377-379. [2]陈秀云;陈春波;吴玉娟;等.;重组基因酵母细胞对DDT类农药的雌激素活性研究[J]
- Methods NOV gene recombinant plasmid was respectively transfected into COS 7 cells and NSCs by liposome method. Conditioned media(CM) of COS 7 cells and COS 7 cells modified by NOV gene were collected. COS CM and NOV CM, NSCs were cultured in CM. 方法 NOV基因重组质粒转染COS 7细胞和NSCs ,收集COS 7细胞和NOV基因修饰COS 7细胞的条件培养液 (简称COS CM和NOV CM ) ,作用于培养的NSCs ,3H TdR掺入检测NSCs的增殖 ,免疫细胞化学和流式细胞仪检测NSCs的分化。
- The result indicates that recombinant PCR is a very effective and reliable method for gene recombination in vitro . 重组 PCR技术是十分有效、可靠的体外基因重组方法
- IBV is a sRNA virus,the gene of the virus always mutated owing to it's point-mutation and gene recombination, so the virus have many serotypes. IBV是单股RNA病毒,该病毒基因可因点突变和重组而发生变异,故传染性支气管炎病毒血清型较多。
- Crossover/mutation gene recombining algorithms were introduced to ensure the new individual to meet precedence constraints. 在交叉／变异过程中通过设计的基因调整算法确保新个体的合法性，以满足工序约束；
- Now focus on the research of gene recombination anti-tumor polypeptide and the basic research of polypeptide for curing bone fracture、osteoporosis. 近期研究领域侧重于基因重组抗肿瘤多肽和治疗骨折、骨质疏松多肽的应用基础研究。
- The antibody antihalf-antigen and antigenic can be mass-produced by gene recombination and used in RII for clinical immunologic diagnosis and scientific researches. 利用基因重组技术大量生产的任何一种相应半抗原和抗原的抗体片段,与RII技术联合,已应用于临床免疫诊断和科学研究。
- Gene recombination and gene transfection are the new progresses in molecular biology,which provides theory and technology basis for further study of tissue engineering. 基因重组、基因转染是分子生物学研究的新进展,为组织工程的深入发展提供理论和技术基础。
- Gene recombinant protein drug produced by animal cell line 动物细胞表达的基因重组蛋白质药物
- Keywords Rickettsia prowazekii;Gene recombinant;Surface antigen; 普氏立克次体;基因重组;表面抗原;
- Keywords Gene recombinant protein;Refolding;Philosophy ; 基因重组蛋白质;复性;哲学;
- Keywords KSR Eukaryotic expression Gene recombinant; 真核表达;基因重组;
- Keywords hepatitis B virus;gene recombinant;eukaryote expression; 肝炎病毒;乙型;基因重组;真核表达;
- This code can be applicable to the design, construction and acceptance of newly built, rebuilt and enlarged biosafety laboratories that used for microbiology, biomedicine, gene recombination and biological produce. 本规范适用于微生物学、物医学、物实验、因重组以及生物制品等使用的新建、建、建的生物安全实验室的设计、工和验收。
- INTERVENTIONS:COS 7 cells were transfected by NOV gene recombined plasmid. COS 7 cell and COS 7 cell modified by NOV gene conditioned culture media(COS CM and NOV CM) were collected and reacted with the cultured HNSCs. 干预:NOV基因重组质粒转染COS-7细胞,收集COS-7细胞和NOV基因修饰COS-7细胞的条件培养液(COS-CM和NOV-CM),作用于培养的HNSCs。
- METHODS: The shRNA specific to PTI-1 (PC-3) was synthesized and inserted into pEGFP/U6 by gene recombination technology to construct the expression vector, pEGFP/U6-mPs, which was then transfected into the cultured PC-3 cells. 方法:采用基因克隆技术,将合成的短发卡样特异性PTI1(PC3)RNA干扰寡核苷酸序列插入真核表达载体pEGFP/U6,构建PTI1(PC3)shRNA的真核表达载体,体外转染人前列腺癌PC3细胞,48h后观察细胞生物学变化;
- OBJECTIVE: To compare the efficacy and safety of China made gene recombined Streptokinase(rSK)and German made streptokinase(SK)in the treatment of acute myocardial infarction(AMI). 对比分析国产基因重组链激酶和德国链激酶静脉溶栓治疗急性心肌梗塞（AMI）的有效性和安全性。
- Meanwhile, the applications of gene recombination technology and cell culture technology in preparation of resveratrol were explained systematically, and its application prospect was also viewed. 同时对基因重组技术、细胞培养技术在白藜芦醇制备方面的应用作了系统阐述,并对其应用前景进行了评价。
