Fermentation curves were investigated by using 7L bioreactor under the optimum conditions. By contrast with the parent strain, the ethanol yield and the Alcohol Dehydrogenase (ADH) activity of HBF-12 decrease 73.6% and 74%, respectively.

• 最优条件下用7L发酵罐进行放大实验；发酵曲线显示:与亲株相比；突变株HBF-12的乙醇产量与乙醇脱氢酶(ADH)活力分别下降了73.；6%25与74%25；乳酸产量与乳酸脱氢酶(LDH)活力分别提高了39